Dextran Sulfate Sodium (DSS)-Induced Colitis Model
Dextran Sulfate Sodium (DSS)-Induced Colitis Model
Huateng Bio offers validated DSS-induced colitis models in Wistar rats for IBD research. Features 90% induction success, DAI scoring, and tight junction protein analysis. Download protocols and validation data.
Model Description
Dextran sulfate sodium (DSS)-induced colitis is a gold-standard preclinical model for studying inflammatory bowel disease (IBD), replicating key features of human ulcerative colitis. Our model provides:
- Controlled inflammation: Dose-dependent mucosal damage and immune activation
- Clinical relevance: Mimics epithelial barrier dysfunction and neutrophil infiltration
- High reproducibility: 90% success rate in inducing acute colitis
Key Applications:
✓ Anti-inflammatory drug screening (5-ASA analogs, biologics)
✓ Gut microbiome interaction studies
✓ Mucosal healing mechanism research
Applications
• IBD therapeutic development
• Nutraceutical efficacy evaluation
• Immune cell trafficking analysis
• Epithelial regeneration studies
Modeling Protocol —— Acute Colitis Induction
1. Group Design:
- Control: Standard drinking water
- Drug Control: Test compound + standard water
- DSS Model: 5% DSS (MW 36-50 kDa) in drinking water
2. Procedure:
- Days 1-5: DSS administration (fresh solution daily)
- Days 6-7: Recovery phase (optional)
- Humane endpoints: Weight loss >20% ∙ Bloody stool score ≥2
Validation & Testing
|
Category |
Parameters |
|
Clinical Observation |
• Disease Activity Index (DAI): Weight loss, stool consistency, bleeding |
|
Hematological Analysis |
CBC ∙ CRP ∙ ESR ∙ Fecal calprotectin ELISA |
|
Histopathology |
H&E staining (colon): Crypt distortion ∙ Ulceration scoring (0-4 scale) |
|
Immunohistochemistry |
CD68+ macrophage infiltration ∙ Occludin/ZO-1 tight junction protein analysis |
|
Cytokine Profiling |
IL-6, TNF-α, IL-1β multiplex assay (Luminex®) |
Technical Advantages
|
Feature |
Our Model |
Alternative Models |
|
Induction Consistency |
90% success rate |
60-70% variability |
|
Disease Progression |
5-day acute phase |
Chronic models requiring weeks |
|
Translational Output |
Direct IBD drug correlation |
Limited clinical relevance |
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