Gastroesophageal Reflux Disease (GERD) Model

Model Description

Gastroesophageal reflux disease (GERD) is characterized by pathological reflux of gastroduodenal contents into the esophagus, leading to mucosal erosion and extra-esophageal complications. Our pyloric ligation model replicates human GERD through:

• Controlled reflux induction: 85% esophagitis incidence rate
• Intact mucosal repair mechanism: Mimics chronic-recurrent disease progression
• High survival rate: >90% (via stent-assisted pyloric ligation)

 Key Advantages:
✓ Surgical precision: 1.5mm stent ensures consistent pyloric narrowing (no complete obstruction)
✓ Dual ligation system: Forestomach ligation reduces gastric volume by 50%
✓ Human-like pathology: Basal cell hyperplasia & inflammatory infiltration (Hetzel-Dent score ≥Grade 2)

Applications

• Acid suppression drug evaluation (PPIs, H2 blockers)
• Mucosal protectant screening (alginate-based formulations)
• Surgical anti-reflux device testing
• Chronic cough/GERD correlation studies

Modeling Protocol —— 7-Day Induction Workflow

1. Pre-op Preparation:

• 24h fasting with ad libitum water access
• Baseline endoscopy recording

 2. Surgical Procedure:

• Midline laparotomy under isoflurane anesthesia
• Pyloric ligation: Silicone stent-assisted 6-0 suture (1.5mm lumen preservation)
• Forestomach ligation: 4-0 silk sutures to reduce gastric capacity

3. Post-op Care:

• Daily weight monitoring (humane endpoint: >20% loss)
• Omeprazole (10mg/kg) analgesia

Validation & Testing

Success Criteria
✓ Endoscopic confirmation of ≥Grade 2 esophagitis
✓ Ulcer index ≥5 (0-8 scale) at Day 7