PCI-Induced Acute Myocardial Infarction Model

Model Description

The minipig’s coronary anatomy and hemodynamic profile closely mirror humans, making it the gold-standard species for AMI studies. Our interventional PCI model replicates clinical AMI through:

• Controlled ischemia: 90-minute LAD occlusion via balloon inflation
• Reperfusion injury: Validated ST-segment elevation (≥2mm ECG shift)
• Cost-effective methodology: Repurposed clinical-grade catheters/guidewires

Clinical Relevance:
✓ Human-like infarct size: 20-30% of left ventricular mass
✓ Translational drug testing: Validated with antiplatelet agents (e.g., ticagrelor)

Applications

• Cardioprotective drug evaluation (ischemia-reperfusion injury therapies)
• Stent/biomaterial safety testing
• Cardiac imaging technology validation (MRI/PET)
• Stem cell therapy mechanism studies

Modeling Protocol —— PCI-Based LAD Occlusion

1. Pre-op Preparation:

• Femoral artery access: 6-Fr radial sheath insertion under ultrasound guidance
• Coronary angiography: Confirm LAD anatomy

2. Balloon Occlusion:

• Position balloon catheter in distal LAD
• Inflate to 6-8 atm for 90-minute ischemia
• Deflate for reperfusion → Remove catheter system

3. Post-op Care: Femoral artery ligation → Analgesia (buprenorphine 0.05mg/kg)

Validation & Testing

Technical Advantages