Pre-Eclampsia (PE) Pregnancy Model
Pre-Eclampsia (PE) Pregnancy Model
Huateng Bio's CD-1 mouse PE model replicates placental insufficiency via TLR activation (R848) or endotoxin induction (LPS). Validates hypertension, proteinuria, and elevated sFlt-1/sEng for antihypertensive drug screening and maternal-fetal immunology studies. GD12.5-GD18.5 protocols available.
Model Description
Pre-eclampsia is a multi-organ pregnancy disorder characterized by placental insufficiency, maternal endothelial dysfunction, and fetal growth restriction. Our chemically induced PE models replicate key clinical hallmarks:
• Hypertension
• Proteinuria
• Elevated anti-angiogenic factors
Pathogenesis Validation
Dual induction protocols available:
1. Toll-like receptor activation: Intraperitoneal R848 injection
2. Endotoxin-mediated inflammation: Tail vein LPS administration
Applications
• Antihypertensive drug screening for PE management
• Placental angiogenesis mechanism studies
• Biomarker discovery (sFlt-1/sEng pathway analysis)
• Maternal-fetal interface immunology research
Modeling Protocols
Method 1: R848 Induction
• Gestational day 12.5: Single intraperitoneal injection of 50 μg R848 (TLR7/8 agonist)
• Monitoring: Daily blood pressure until GD18.5
Method 2: LPS Induction
• Gestational day 14: Tail vein injection of 50 μg/kg LPS
• Endpoint analysis at GD18.5
Validation & Endpoints
|
Category |
Parameters |
|
Hemodynamic |
Tail-cuff systolic BP ∙ Mean arterial pressure (MAP) monitoring |
|
Renal Function |
24-hour urinary protein ∙ Urinary creatinine ratio |
|
Fetal Outcomes |
Fetal weight ∙ Placental efficiency index ∙ Resorption rate calculation |
|
Serum Biomarkers |
sFlt-1/sEng ELISA ∙ PIGF immunoassay ∙ TNF-α/IL-6 cytokine profiling |
|
Histopathology |
Placental H&E staining ∙ Renal glomerular endotheliosis scoring |
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