Subcutaneous Tumor Model

Model Description

Subcutaneous tumor models are commonly used to study the formation and development of tumors, as well as to evaluate tumor treatment strategies. To better mimic human tumor diseases, human-derived tumor cell lines are often selected. Due to the potential for immune rejection in xenograft models, immunodeficient animals are preferred for research.

Nude mice, which lack a functional immune system, are a popular choice in research due to their unique characteristics. The subcutaneous tumor model typically involves injecting human tumor cells under the skin of nude mice to form visible tumors, allowing for the observation of tumor growth and biological characteristics. 

Applications

Assess the efficacy of antitumor drugs, immunotherapies, and other novel therapeutic strategies.

Modeling Methods

1. Cell Culture: Collect HeLa cervical cancer cells in the logarithmic growth phase, prepare a cell suspension using enzymatic digestion, and adjust the concentration of the cell suspension as needed.

2. Inoculation: Inject 3×10^6 cells per mouse into the subcutaneous tissue of BALB/c-nu nude mice (insert the needle approximately 1 cm into the subcutaneous tissue on the shoulder).

This establishes a subcutaneous cervical cancer model.

Testing items

• Measure the long diameter (a) and short diameter (b) of the tumor every 3 days using calipers 
• Calculate the tumor volume (TV)