Ear, nose and throat (ENT) models

Model Description

 The procedure involves viral injection into the posterior semicircular canal of mice to simulate ear-related pathologies.

Modeling Methods

1. Anesthetize the mouse and prepare the surgical site by shaving and disinfecting the skin.

2. Make an incision along the edge of the ear, separate the subcutaneous tissue, and expose the facial nerve and sternocleidomastoid muscle through blunt dissection.

3. Locate the posterior semicircular canal behind the facial nerve and isolate it by dissecting the overlying muscles.

4. Use an insulin syringe to create a small hole in the posterior semicircular canal and insert a polyamide tube connected to a microinjection pump.

5. Inject the virus into the posterior semicircular canal and allow it to sit for 5 minutes before removing the tube.

6. Seal the hole with a small piece of muscle, suture the skin, and monitor the mouse until it wakes up.

7. Place the mouse on a warming pad to recover body temperature and administer intramuscular penicillin to prevent infection.

Testing Items:

• ABR (Auditory Brainstem Response) at 4, 8, and 16 kHz
• Histopathology: Immunohistochemistry and immunofluorescence of cochlear tissue sections