Hemorrhagic Shock Model

Applications

• Evaluation of novel hemostatic agents and wound dressings.

• Testing of fluid resuscitation protocols (crystalloids, colloids, blood products).

• Research on hemorrhagic shock-induced Multiple Organ Dysfunction Syndrome (MODS).

• Validation of medical devices for emergency trauma care.

Modeling Methods

HuaTeng Biotechnology excels in two gold-standard induction protocols:

• Pressure-Controlled (Wiggers’ Model): Withdrawal of blood to maintain Mean Arterial Pressure (MAP) at a target level (e.g., 35-40 mmHg) for a specific duration.

• Fixed-Volume Hemorrhage: Removal of a pre-calculated percentage (30-50%) of total blood volume.

• Polytrauma Combination: HS combined with soft tissue injury or bone fracture to simulate complex trauma scenarios.

Testing Items

• Hemodynamics: Continuous monitoring of MAP, Heart Rate, and Cardiac Output (CO).

• Metabolic Profiling: Blood gas analysis (pH, Lactate, Base Excess) to monitor tissue perfusion.

• Organ Function: Serum Cr/BUN (Kidney), ALT/AST (Liver), and Myocardial enzymes.

• Coagulation Profile: TEG (Thromboelastography) or ROTEM to assess Trauma-Induced Coagulopathy (TIC).

• Inflammatory Markers: Plasma levels of TNF-α, IL-1β, and HMGB1.

The porcine model is the international gold standard for HS research due to its comparable blood volume and compensatory vascular responses. This allows researchers to use clinical-grade ventilators, infusion pumps, and invasive monitoring equipment.