Oncology

Cell Line-Derived Xenograft (CDX) Model

• Subcutaneous or orthotopic implantation of immortalized cancer cell lines (e.g., U87, A549) into immunodeficient mice.

Patient-Derived Xenograft (PDX) Model

• Direct implantation of fresh patient tumor tissue into immunodeficient mice, preserving tumor heterogeneity and stroma.

VX2 Tumor Model

• A transplantable rabbit squamous cell carcinoma line, widely used in liver, bone, and interventional oncology research.

Glioma Model

• Orthotopic Xenograft: Intracranial injection of glioma cells (e.g., U87, GL261).
• Transgenic/GENETIC Models: (e.g., RCAS/tv-a system).

Breast Cancer Model

• Orthotopic Xenograft: Mammary fat pad injection of cells (e.g., MDA-MB-231, 4T1).
• Spontaneous Models: MMTV-PyMT transgenic mice.

Liver Cancer Model

• Chemical Induction: Diethylnitrosamine (DEN) injection.
• Orthotopic Xenograft: Intrasplenic or intrahepatic injection of cells (e.g., HepG2, Huh7).

Colon Cancer Model

• Chemical Induction: Azoxymethane (AOM) with Dextran Sulfate Sodium (DSS).
• Orthotopic Xenograft: Cecal or colonic wall injection of cells (e.g., HCT116, CT26).

Gastric Cancer Model

• Chemical Induction: N-Methyl-N-nitrosourea (MNU) in drinking water.
• Xenograft: Subcutaneous or orthotopic implantation of cells (e.g., MKN-45).

Prostate Cancer Model

• Subcutaneous/Orthotopic Xenograft: Injection of cells (e.g., PC-3, LNCaP).
• Transgenic Models: TRAMP mice.

Cervical Cancer Model

• Subcutaneous Xenograft: Injection of HPV-positive cell lines (e.g., HeLa, SiHa) in immunodeficient mice.

Skin Cancer Model

• Chemical Induction: DMBA/TPA two-stage carcinogenesis protocol.
• UV-Induced Carcinogenesis.

Disease Model

Modeling Method / Key Information

Cancer Metastasis Model

• Experimental Metastasis: Direct intravenous (e.g., tail vein) injection of tumor cells to seed lungs/liver.
• Spontaneous Metastasis: Orthotopic implantation of highly invasive cells, allowing primary tumor growth and natural metastasis.

Tumor Model (by Injection Site)

• Subcutaneous Injection: For convenient tumor growth monitoring and drug efficacy screening.
• Orthotopic Injection: Implantation into the organ/tissue of origin, providing a more relevant tumor microenvironment.
• Tail Vein Injection: Primarily for establishing experimental metastasis models (e.g., lung colonization).
• Intracranial Injection: For modeling brain tumors or brain metastases.