Hindlimb Ischemia Model
Hindlimb Ischemia Model
HuaTeng Biotechnology provides high-fidelity Hindlimb Ischemia (HLI) models for PAD research. Expert femoral artery ligation in swine and rats with Laser Doppler Blood Flow (LDBF) validation and IHC analysis.
Applications
• Preclinical efficacy testing of pro-angiogenic factors and cell-based therapies.
• Validation of interventional devices for peripheral vascular revascularization.
• Molecular research on ischemia-induced muscle remodeling and collateral growth.
Modeling Methods
Precision Surgical Induction:
• Small Animals: Standardized excision of the femoral artery from the inguinal ligament to the popliteal bifurcation to minimize spontaneous collateral bypass.
• Large Animals (Swine): Clinically relevant ischemia induced via permanent surgical ligation or endovascular coil embolization.
Testing Items
• Perfusion Analysis: Longitudinal Laser Doppler Blood Flow (LDBF) imaging to quantify blood flow recovery ratios.
• Microvascular Quantification: IHC for CD31 (Capillary density) and α-SMA (Arteriolar density).
• Histomorphometry: Capillary-to-muscle fiber ratio, H&E for myofiber atrophy, and Masson’s for interstitial fibrosis.
• Functional Testing: Graded treadmill exercise and limb salvage scoring.
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