Autoimmune Myocarditis Model

Modeling Process at HuaTeng 

We ensure the highest level of reproducibility through standardized SOPs:

1. Antigen Preparation: Precise emulsification of cardiac myosin with adjuvant.

2. Systemic Immunization: Multi-point subcutaneous injection to trigger a robust immune response.

3. Longitudinal Monitoring: Regular body weight tracking and clinical symptom observation.

4. Endpoint Analysis: Comprehensive cardiac assessment at the peak of inflammation or chronic phase.

Validation & Evaluation 

HuaTeng Biotechnology provides a complete data package for every EAM project:

• Cardiac Function (In Vivo): High-resolution Echocardiography measuring EF% (Ejection Fraction) and FS% (Fractional Shortening) to quantify systolic dysfunction.

• Inflammatory Biomarkers: Serum analysis of cTnI/cTnT, NT-proBNP, and cytokines (IL-6, TNF-α, IFN-γ) via ELISA.

• Histopathological Analysis (The Gold Standard): * H&E Staining: To evaluate the severity of inflammatory cell infiltration and myocyte necrosis.

  • Masson’s Trichrome Staining: To quantify interstitial fibrosis in the chronic phase.

  • Immunohistochemistry (IHC): Detection of CD3+, CD4+, and CD68+ cell infiltration.

Why HuaTeng Biotechnology for Cardiovascular Research?

• Vast Experience: We have successfully delivered hundreds of cardiovascular models, including a proven track record of 200+ rat models and 100+ large animal (swine) models.

• Multidisciplinary Team: Our experts combine pharmacology, cardiac surgery, and pathology to ensure your study’s success.

• Customization: We can adapt the EAM model to focus on the acute inflammatory phase or the progression into Dilated Cardiomyopathy (DCM) based on your drug's mechanism of action.

• Global Standard: Facilities and protocols designed to meet international R&D requirements for pharmaceutical and biotech clients.